Chemistry: molecular biology and microbiology – Vector – per se
Patent
1985-03-27
1988-11-08
Martinell, James
Chemistry: molecular biology and microbiology
Vector, per se
435 68, 435 70, 435 71, 435 91, 4351721, 4351723, 435253, 536 27, 935 9, 935 10, 935 14, 935 29, 935 47, 935 58, C12N 1500, C12N 120, C12P 2100, C12P 2102, C12P 2104, C12P 1934, C07H 2104
Patent
active
047834153
ABSTRACT:
A DNA segment of a gene coding for a signal peptide is disclosed the segment is to be used in a host-vector system employing a bacterium belonging to the genus Streptomyces as a host by incorporation in the vector DNA. The segment comprises a DNA having a base sequence coding for the peptide having the amino acid sequence from X to Y in the annexed FIG. 1.
A vector plasmid to be used for transformation of bacteria belonging to the genus Streptomyces is also disclosed. The plasmid comprises a DNA segment with a base sequence coding for an extra-cellular .alpha.-amylase derived from Streptomyces hygroscopicus SF-1084 strain or a mutant thereof the DNA segment incorporated into a vector plasmid is to be used in a host-vector system employing a bacterium belonging to the genus Streptomyces as a host and also having a DNA segment necessary for expression of the gene information of the DNA segment.
The present invention may be applied to the production of a desired protein which will be secreted outside the host microorganism. .
REFERENCES:
patent: 4338400 (1982-07-01), Manis et al.
European Search Report-EP 85 30 2177.
Gene, vol. 29, No. 3, Sep. 1984, pp. 315-321, Elsevier Science Publishers, Amsterdam, NL; K. Kendall et al.: "Cloning and Expression of an Extracellular-Agarase Gene from Streptomyces coelicolor A3(2) in Streptomyces lividans 66".
Nucleic Acids Research, vol. 12, No. 13, Jul. 1984, pp. 5145-5164, IRL Press Ltd., Oxford, GB; M. E. E. Watson: "Compilation of Published Signal Sequences".
Hidaka et al., Chem. Abstr. 95: 20240t (1981).
Palva et al, Proc. Natl. Acad. Sci. USA 79: 5582 (1982).
Fukatsu Shunzo
Hoshiko Shigeru
Makabe Osamu
Nagaoka Kozo
Martinell James
Meiji Seika Kabushiki Kaisha
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