Fluorescent protein sensors for measuring the pH of a biological

Details Fluorescent protein sensors for measuring the pH of a biological Fluorescent protein sensors for measuring the pH of a biological

1998-06-09

2000-10-31

Slobodyansky, Elizabeth

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

530350, G01N 3352, C07K 14435

Patent

active

061401323

ABSTRACT:
Disclosed are fluorescent protein sensors for measuring the pH of a sample, nucleic acids encoding them, and methods of use. The preferred fluorescent protein sensors are variants of the green fluorescent protein (GFP) from Aequorea victoria. Also disclosed are compositions and methods for measuring the pH of a specific region of a cell, such as the mitochondrial matrix or the Golgi lumen.

REFERENCES:
Llopis et al. (Jun. 9, 1998) Proc. Natl. Acad. Sci. USA, vol. 95, pp. 6803-6808.
Kneen et al. (Mar. 1998) Biophysical J., vol. 74, pp. 1591-1599 (abstract).
Guttenplan et al. (1973) BBA, vol. 322, pp. 294-300 (abstract).0.

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