Fluorescent assay

Details Fluorescent assay Fluorescent assay

2011-08-16

2011-08-16

Wallenhorst, Maureen M (Department: 1777)

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

Glycosylated hemoglobin

C436S063000, C436S066000, C436S164000, C436S172000, C422S052000, C422S082050, C422S082080

Reexamination Certificate

active

07998742

ABSTRACT:
A method and apparatus to estimate the concentration of a non-fluorescent substance (e.g. haemoglobin) in a fluorescent assay by separately estimating the non time dependent alteration attributed to inherent filter effects from the time dependent alteration caused by the assay chemistry. Such a method obviates the requirement for a separate photometric or other measurement thereby simplifying the methodology and associated instrumentation.

REFERENCES:
patent: 4861728 (1989-08-01), Wagner
patent: 5242842 (1993-09-01), Sundrehagen
patent: 5478754 (1995-12-01), Brandt et al.
patent: 5877025 (1999-03-01), Edwards et al.
Mertens et al. Analytical Biochemistry, vol. 96, issue 2, Jul. 15, 1979, pp. 448-455.
Blincko et al. (abstract) Annals of Clinical Biochemistry, vol. 37, No. 3, pp. 372-379, 2000.

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