Flow cytometric, whole blood dendritic cell immune function...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007200, C435S040500, C435S287200, C435S962000, C435S973000, C436S010000, C436S017000, C436S063000, C436S064000, C436S164000, C436S172000, C436S177000, C436S522000, C436S546000

Reexamination Certificate

active

10635972

ABSTRACT:
The invention provides a flow cytometric method for measuring dendritic cell function in whole blood, comprising: (a) contacting a whole blood sample with a dendritic cell activator; (b) adding to the sample a plurality of dendritic cell-distinguishing antibodies and at least one cytokine-specific antibody; and then (c) flow cytometrically assaying the sample for the binding of the cytokine-specific antibody by at least one distinguishable DC subset. Other assays are presented in which DC activation markers are assessed.

REFERENCES:
patent: 4654312 (1987-03-01), Chang et al.
patent: 4902613 (1990-02-01), Chang et al.
patent: 5098849 (1992-03-01), Hilerio et al.
patent: 5597563 (1997-01-01), Beschorner
patent: 5627025 (1997-05-01), Steinman et al.
patent: 5627040 (1997-05-01), Bierre et al.
patent: 5643786 (1997-07-01), Cohen et al.
patent: 5648219 (1997-07-01), MacKay et al.
patent: 5648248 (1997-07-01), Zenke et al.
patent: 5698679 (1997-12-01), Nemazee
patent: 5788963 (1998-08-01), Murphy et al.
patent: 6495333 (2002-12-01), Willmann et al.
patent: WO 97/24438 (1997-07-01), None
patent: WO 97/29183 (1997-08-01), None
patent: WO 98/15579 (1998-04-01), None
patent: WO 98/15615 (1998-04-01), None
Olweus et al., Peripheral Blood Dendritic Cells Revealed by Flow Cytometry, Becton Dickinson—Application Note 3, (1998).
Becton Dickinson, Detection of Intracellular Cytokines in Activated LymphocytesApplication Note 1, (1996).
Andersson, U. et al., “Enumeration of IFN-.gamma.-Producing Cells by Flow Cytometry,” J. Immunological Methods, 112 (1) :139-142 (1988).
Banchereau, J. et al., “Dendritic Cells and the Control of Immunity,” Nature, 392:245-252 (1998).
Becton Dickinson Immunocytometry Systems, “Detection of Intracellular Cytokines in Activated Lymphocytes,” Application Note 1, Immune Function, 1-12 (1996).
Caux, C. et al., “CD34+ Hematopoietic Progenitors from Human Cord Blood Differentiate Along Two Independent Dendritic Cell Pathways in Response to GM-CSF+TNF.alpha.,” J. Exp. Med., 184 (2): 695-706 (1996).
Caux, C. et al., “Tumor Necrosis Factor-alpha Strongly Potentiates Interleukin-3 and Granulocyte-Macrophage Colony-Stimulating Factor-Induced Proliferation of Human CD34.sup.+ Hematopoietic Progenitor Cells,” Blood, 75 (12) :2292-2298 (1990).
deCaestecker, M.P. et al., “The Detection of Intracytoplasmic Interleukin-1.alpha., Interleukin-1.beta. and Tumor Necrosis Factor .alpha. Expression in Human Monocytes Using Two Colour Immunofluorescence Flow Cytometry,” J. Immunological Methods, 154 (1) :11-20 (1992).
de Saint-Vis, B. et al., “The Cytokine Profile Expressed by Human Dendritic Cells Is Dependent on Cell Subtype and Mode of Activation,” J. Immunology, 160:166-1676 (1998).
Ghanekar, S. et al., “Cytokine Expression by Human Peripheral Blood Dendritic Cells Stimulated In Vitro with HIV-1 and Herpes Simplex Virus,” J. Immunology, 157 (9) :4028-4036 (1996).
Grouard, G. et al., “The Enigmatic Plasmacytoid T Cells Develop into Dendritic Cells with Interleukin (IL) -3 and CD40-Ligand,” J. Exp. Med., 185 (6) :1101-1111 (1997).
Grouard, G. et al., “Role of Dendritic and Follicular Dendritic Cells in HIV Infection and Pathogenesis,” Current Opinion in Immunology, 9 (4) :563-567 (1997).
Hallden, G. et al., “A New Membrane Permeabilization Method for the Detection of Intracellular Antigens by Flow Cytometry,” J. Immunological Methods, 124 (1) :103-109 (1989).
Ingalls, R.R. et al., “CD11c/CD18, A Transmembrane Signaling Receptor for Lipopolysaccharide,” J. Exp. Med., 181 (4) : 1473-1479, 1998.
Jacob, M.C. et al., “Membrane Cell Permeabilisation With Saponin and Mutiparametric Analysis by Flow Cytometry,” Cytometry, 12 (6) :550-558 (1991).
Jung, T. et al., “Detection of Intracellular Cytokines by Flow Cytometry,” J. Immunological Methods, 159:197-207 (1993).
Kabilan, L. et al., “Detection of Intracellular Expression and Secretion of Interferon-.gamma. at the Single-Cell Level After Activation of Human T Cells with Tetanus Toxoid In Vitro,” European J. Immunology, 20 (5) :1085-1089 (1990).
Kahan, Mel, “Detecting Intracellular Cytokines in Activated Monocytes,” Application Note 2, Immune Function, Becton Dickinson Immunocytometry Systems, 1-12 (1997).
Lore K. et al., “Immunocytochemical Detection of Cytokines and Chemokines in Langerhans Cells and In Vitro Derived Dendritic Cells,” J. Immunological Methods, 214:97-111 (1998).
Macatonia, S.E. et al., “Dendritic Cells Produce IL-12 and Direct the Development of Th1 Cells from Naive CD4.sup.30 T Cells,” J. Immunology, 154 (10):5071-5079 (1995).
Maino, V.C. et al., “Flow Cytometric Method for Analysis of Cytokine Expression in Clinical Samples,” Clinical Immunology Newsletter, 16(6) :95-98 (1996).
O'Doherty, U. et al., “Human Blood Contains Two Subsets of Dendritic Cells, One Immunologically Mature and the Other Immature,” Immunology, 82 (3) :487-493 (1994).
Olweus, J. et al., “Dendritic Cell Ontogeny: A Human Dendritic Cell Lineage of Myeloid Origin,” Proc. Natl. Acad. Sci. USA, 94 (23) :12551-12556 (1997).
Picker, L.J. e al., “Direct Demonstration of Cytokine Synthesis Heterogeneity Among Human Memory/Effector T Cells by Flow Cytometry,” Blood, 86 (4) :1408-1419 (1995).
Prussin, C., Cytokine Flow Cytometry: Assessing Cytokine Production at the Single Cell level, Clinical Immunology Newsletter, 16 (6) : 85-91 (1996).
Sander, B. et al., “Assessment of Cytokines by Immunofluorescence and the Paraformaldehyde-Saponin Procedure,” Immunological Reviews, 119:65-93 (1991).
Scheinecker, C. et al., “Phenotypic, Morphological and Functional Analysis of CD11c.sup.+ and CD11c Human Peripheral Blood Dendritic Cells,” ASH Meeting, San Diego, Dec. 1997.
Steinman, R.M. et al., “Identification of a Novel Cell Type in Peripheral Lymphoid Organs of Mice,” J. Exper. Med., 137:1142-1162 (1973).
Steinman, R.M., “The Dendritic Cell System and its Role In Immunogenicity,” Annu. Rev. Immunol., 9:271-296 (1991).
Suni, M.A. et al., “Detection of Antigen-Specific T Cell Cell Cytokine Expression in Whole Blood by Flow Cytometry,” Journal of Immmunological Methods, 212:89-98 (1998).
Thomas, R. et al., “Human Peripheral Blood Dendritic Cell Subsets,” J. Immunology, 153 (9) :4016-4028 (1994).
Vikingsson, A. et al., “Enumeration of IFN-.gamma. Producing Lymphocytes by Flow Cytometry and Correlation with Quantitative Measurement of IFN-.gamma.,” J. Immunological Methods, 173 (2) : 219-228 (1994).
Waldrop, S.L. et al., “Determination of Antigen-specific Memory/Effector CD4.sup.+ T Cell Frequencies by Flow Cytometry,” J. Clinical Investigation, 99 (7) :1739-1750 (1997).
Weissman, D. et al., “Role of Dendritic Cells in Immunopathogenesis of Human Immunodeficiency Virus Infection,” Clinical Microbiology Reviews, 10 (2) :358-367 (1997).
Willmann, K. et al., “Peripheral Blood Dendritic Cells Revealed by Flow Cytometry,” Application Note 3, Reagents, Becton Dickinson Immunocytometry Systems, 1-12 (1998).
Zhou, L. et al., A Distinct Pattern of Cytokine Gene Expression by Human CD83.sup.+ Blood Dendritic Cells, Blood, 86 (9) : 3295-3301 (1995).
Zoeteweij, J.P. et al., HIV-Dendritic Cell Interactions Promote Efficient Viral Infection of T Cells, J. Biomed. Sci., 5:253-259 (1998).
D. Becker et al., “Flow-cytometric screening for the modulation of receptor-mediated endocytois in human dendritic cells: implications for the development of an in vitro technique for predictive testing of contact sensitizers”, Journal of Immunological Methods, vol. 203, No. 2, pp. 171-180 (Apr. 25, 1997).

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