Fibrin binding domain polypeptides and uses and methods of produ

Details Fibrin binding domain polypeptides and uses and methods of produ Fibrin binding domain polypeptides and uses and methods of produ

1997-08-11

2000-09-19

Sisson, Bradley

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Chemical modification or the reaction product thereof, e.g.,...

424 91, 424 94, 424 169, 530381, 530380, 435 691, 4353201, 4352523, 435 76, 435 71, 435 4, C07K 1107

Patent

active

061214264

ABSTRACT:
This invention provides an imaging agent which comprises a polypeptide labeled with an imageable marker, such polypeptide having an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and being capable of binding to fibrin. The invention further provides a method wherein the imaging agent is used for imaging a fibrin-containing substance, i.e., a thrombus or atherosclerotic plaque. Further provided are plasmids for expression of polypeptides having an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and being capable of binding to fibrin, hosts containing these plasmids, methods of producing the polypeptides, methods of treatment using the polypeptides, and methods of recovering, refolding and reoxidizing the polypeptides. The invention also provides for purified polypeptides substantially free of other substances of human origin which have an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and which are capable of binding to fibrin.

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