Expression vector encoding a triterpene hydroxylase polypeptide

Details Expression vector encoding a triterpene hydroxylase polypeptide Expression vector encoding a triterpene hydroxylase polypeptide

2005-02-25

2009-08-18

Steadman, David J (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing a...

C435S189000, C435S320100, C435S069100, C435S252300, C435S254110, C435S254200, C536S023200, C536S023600

Reexamination Certificate

active

07575892

ABSTRACT:
Soyasapogenol B is biosynthesized via two steps of hydroxylation reaction of its precursor β-amyrin. However, the gene of the hydroxylase concerned in this reaction has not been revealed. Therefore, it was impossible to apply a genetic engineering technique on the hydroxylase. The present inventors reveals that a sequence which corresponds to a soybean-derived cytochrome P-450 gene CYP93E1 encodes an enzyme protein that carries out hydroxylation of the 24-position of an oleanane type triterpene, and also provides a method for applying said gene making use of a genetic engineering technique.

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