Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an...
Patent
1989-06-30
1994-01-18
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an...
435177, 435180, 435181, 436524, 436528, 436531, 436532, 530811, 530812, 530815, 530816, C12N 1114, C12N 1102, G01N 33551, G01N 33544
Patent
active
052799549
ABSTRACT:
The invention provides a means for attaching a label, support or bioactive agent to a protein with an exopeptidase at a site that is remote from the active site of the protein. More specifically the invention is directed to a method for the attachment of an amino acid, amine and alcohol nucleophile to the carboxyl terminus of a protein. In one embodiment, a labeled nucleophile is attached to a protein such as an antibody. In other embodiments, the invention is directed to a method for the attachment of a protein to an immobilization support and to a method for the attachment of a bioactive agent to a protein.
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Coolidge Thomas R.
Lewis William
Schuster Sheldon M.
Stout Jay
Wagner Fred W.
Board of Regents of the University of Nebraska and BioNebraska
Naff David M.
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