Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1986-05-29
1989-11-14
Teskin, Robin
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 70, 4351723, 435 91, 435320, 435255, 435256, 536 27, 935 28, 935 37, 935 41, 935 56, 935 60, C12P 2100, C12P 2102, C12N 500, C12N 1500
Patent
active
048807346
ABSTRACT:
Methods and compositions are provided for efficient expression of genes in unicellular microoorganisms, particularly yeast. The systems involve an expression system employing transcriptional initiation regions from glycolytic enzymes, particularly a chimeric expression system, having a first region providing for regulatable or constitutive expression, a second region providing for transcriptional initiation, where regions one and two are not found joined together in functional relationship in nature, and optionally a sequence providing for a secretory leader and processing signal, where the expression cassette will be joined to a gene which may be homologous or heterologous to the host. The expression cassette can be used on an extrachromosomal element or integrated into the host genome, whereby continuous expression can be achieved or inducible expression is obtained, by virtue of the presence or absence of an inducer. Constructions may be provided, where structural genes may be introduced in reading frame with the cassette to provide for expression of the natural peptide, a fused peptide, or a peptide precursor.
The S. cerevisiae strain AB110 (pAB24/P. vivax 1-5 was deposited at the A.T.C.C. on May 9, 1986, and given Accession No. 20797.
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Barr Philip J.
Burke Rae L.
Rosenberg Steven
Shuster Jeffrey R.
Tekamp-Olson Patricia
Chiron Corporation
Teskin Robin
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