Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1986-04-23
1989-02-28
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 21, 435188, G01N 33535, C12Q 142
Patent
active
048085227
ABSTRACT:
A method for determination of an antigen, which comprises preparation of a onjugate by way of cross-linking of an antibody with an inorganic pyrophosphatase, reacting the resulting conjugate with the antigen to be determined attached to the surface of a solid phase, separating the solid phase from the liquid one, reacting one of said phases with a substrate represented by a salt of pyrophosphoric acid. After carrying out the reaction, a color reagent incorporating a salt of molybdic acid, a surfactant, malachite green dye and a strong inorganic acid is added to the resulting reaction mixture and the content of the antigen is determined from its optical density.
REFERENCES:
patent: 3791932 (1974-02-01), Schuurs
patent: 3879262 (1975-04-01), Schuurs
patent: 3966556 (1976-06-01), Rubenstein
patent: 4157280 (1979-06-01), Halbert
patent: 4190496 (1980-02-01), Rubenstein
patent: 4474878 (1984-10-01), Halbert
Atabekov Iosif G.
Avaeva Svetlana M.
Baikov Alexandr A.
Kasho Vladimir N.
Kulinich Alexandr V.
Benson Robert
Kepplinger Esther M.
Moskovsky Gosudarstvennx Universitet Imeni M. V. Lomonoso VA
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