Enzyme having β-glucosidase activity and use thereof

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S004000, C435S006120, C435S069100, C435S183000, C435S041000, C435S252300, C435S320100, C536S023200, C536S023400, C536S023500, C536S023700, C426S056000, C426S549000

Reexamination Certificate

active

10381434

ABSTRACT:
The invention is to provide a novel β-glucosidase and a gene that codes for the enzyme, and to develop a technique of utilizing the β-glucosidase or a composition that contains the enzyme for processing plants or plant-derived substances.According to the invention, there are provided a novel enzyme showing a β-glucosidase activity and derived from filamentous fungi of the genus Acremonium, a gene that codes for the enzyme, a method of using the gene for expressing β-glucosidase, an enzyme composition that contains β-glucosidase, and a method of processing plants or plant-derived substances with the enzyme or the enzyme compositions.

REFERENCES:
patent: 4562150 (1985-12-01), Yamanobe et al.
patent: 59-166081 (1984-09-01), None
patent: 4-117244 (1992-04-01), None
patent: 7-236431 (1995-09-01), None
patent: 7-264994 (1995-10-01), None
patent: 92/10581 (1992-06-01), None
Takashi Kawaguchi et al.: “Cloning and sequenceing of the cDNA encoding beta-glucosidase 1 fromaspergillus aculeatus” GENE, vol. 173, No. 2, pp. 287-288, 1996.
SM Pitson et al.: “Purification and characterization of an extracellular beta-glucosidase from the filamentous fungus acremonium persicinum and its probable role in beta-gluccan degradation” Enzyme Microb Technol., vol. 21, No. 3, pp. 182-190, Aug. 15, 1997.
Supannee Kansarn et al.: “Purification and characterization of three beta-glucosidases fromacremonium cellulolyticus” Shizuoka Daigaku Daigakuin Denshi Kagaku Kenkyuu-Ka Kenkyuu Houkoku, No. 21, pp. 9-15 Mar. 31, 2000.

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