Enzyme and enzyme preparation with endoglucanase activity

Details Enzyme and enzyme preparation with endoglucanase activity Enzyme and enzyme preparation with endoglucanase activity

1997-03-20

1999-07-06

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352552, 4352561, 4352567, 4353201, 536 232, C12N 942, C12N 1555

Patent

active

059196912

DESCRIPTION:

BRIEF SUMMARY
The present invention relates to an enzyme composition, an isolated enzyme with cellulolytic (endoglucanase) activity at alkaline conditions, a DNA construct encoding the enzyme, a method of producing the enzyme or enzyme composition, a detergent composition comprising the enzyme or the composition, and use of the enzyme e.g. in the detergent industry, the textile industry and the paper pulp industry.


BACKGROUND OF THE INVENTION

Endoglucanases (EC No. 3.2.1.4) constitute a group of hydrolases, which catalyse endo hydrolysis of 1,4-.beta.-D-glycosidic linkages in cellulose, cellulose derivatives (such as carboxy methyl cellulose and hydroxy ethyl cellulose), lichenin, .beta.-1,4 bonds in mixed .beta.-1,3 glucans such as cereal .beta.-D-glucans or xyloglucans and other plant material containing cellulosic parts. The authorized name is endo-1,4-.beta.-D-glucan 4-glucano hydrolase, but the abbreviated term endoglucanase is used in the present specification. Reference can be made to T. -M. Enveri, "Microbial Cellulases" in W. M. Fogarty, Microbial Enzymes and Biotechnology, Applied Science Publishers, p. 183-224 (1983); Methods in Enzymology, (1988) Vol. 160, p. 200-391 (edited by Wood, W. A. and Kellogg, S. T.); Beguin, P., "Molecular Biology of Cellulose Degradation", Annu. Rev. Microbiol. (1990), Vol. 44, pp. 219-248; Beguin, P. and Aubert, J -P., "The biological degradation of cellulose", FEMS Microbiology Reviews 13 (1994) p.25-58; Henrissat, B., "Cellulases and their interaction with cellulose", Cellulose (1994), Vol. 1, pp. 169-196. Celluloses are found in connection with many gums and they are components of cell walls in e.g. fruits, vegetables and cereals.
Endoglucanases have been found to be produced by various types of organisms such as plants and microorganisms, and endoglucanases of a wide variety of specificities have been identified.
Fungal endoglucanases have been described by Sheppard, P.O., et al., "The use of conserved cellulase family-specific sequences to clone Cellulase homologue cDNAs from Fusarium oxysporum, Gene, (1994), Vol. 15, pp. 163-167, Saloheimo, A., et al., "A novel, small endoglucanase gene, egI5, from Trichoderrma reesei isolated by expression in yeast", Molecular Microbiology (1994), Vol. 13(2), pp. 219-228; van Arsdell, J. N. et al, (1987), Cloning, characterization, and expression in Saccharomyces cerevisiae of endoglucanase I from Trichoderma reesei, Bio/Technology 5: 60-64; Penttila, M. et al., (1986), "Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I gene", Gene 45:253-263; Saloheimo, M. et al, (1988), "EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme", Gene 63:11-21; Gonzales, R., et al., "Cloning, sequence analysis and yeast expression of the egll gene from Trichoderma longibrachiatum", Appl. Microbiol. Biotechnol., (1992), Vol. 38, pp. 370-375; Ooi, T. et al. "Cloning and sequence analysis of a cDNA for cellulase (FI-CMCase) from Aspergillus aculeatus", Curr. Genet., (1990), Vol. 18, pp. 217-222; Ooi, T. et al, "Complete nucleotide sequence of a gene coding for Asper-gillus aculeatus cellulase (FI-CMCase)", Nucleic Acids Research, (1990), Vol. 18, No. 19, p. 5884; Xue, G. et al., "Cloning and expression of multiple cellulase cDNAs from the anaerobic rumen fungus Neocallimastix patriciarum in E. coli", J. Gen. Microbiol., (1992), Vol. 138, pp. 1413-1420; Xue, G. et al., "A novel polysaccharide hydrolase cDNA (celD) from Neocallimastix patriciarum encoding three multi-functional catalytical domains with high endoglucanase, cellobiohydrolase and xylanase activities", J. Gen. Microbiol., (1992), Vol. 138, pp. 2397-2403; Zhou, L. et al., "Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase", Biochem. J., (1994), Vol. 297, pp. 359-364; Dalb.ae butted.ge, H. and Heldt-Hansen, H. P., "A novel method for efficient expression cloning of fungal enzyme genes", Mol. Gen. Genet., (1994), Vol. 243, pp. 253-260; Ali, B

REFERENCES:
patent: 4562150 (1985-12-01), Yamanobe et al.
Penicillium and Acremonium, vol. 1, Plenum Press, New York and London (1987), pp. 2-5.
Abstract (K-114)--Malburg et al., The xynB Gene from Fibrobacter succinogenes S85 Encodes a Xylanase which Possesses Endoglucanase and Cellobiosidase Activities (no date).
Chemical Abstracts, vol. 122, No. 3, Abstract No. 26388v (1995).
Dialog Information Services, file 5, Biosis, Dialog Accession No. 10520274, Biosis Accession No. 96120274. (1993).
EMBL, Gen Bank, DDBJ, Accession No. L29378. (1994).
EMBL, Gen Bank, DDBJ, Accession No. D63516. (1995).
EMBL, Gen Bank, DDBJ, Accession No. X76046. (1994).
Chemical Abstracts, vol. 102, No. 7, Abstract No. 60789y (1985).
Dalboge, H., et. al. (1994) Mol. Gen Genet. 243, 253-260.

Affiliated with

Also associated with

Rating

Enzyme and enzyme preparation with endoglucanase activity does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzyme and enzyme preparation with endoglucanase activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzyme and enzyme preparation with endoglucanase activity will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-898662