Enhanced indole biosynthesis

Details Enhanced indole biosynthesis Enhanced indole biosynthesis

1994-06-21

1996-02-27

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

435122, 435 691, 435 711, 435156, 4351723, 4352402, 4352523, 43525233, 43525234, 4353201, 536 221, 536 231, 536 232, 536 237, C12N 900, C12N 120, C12P 722, C12P 1710

Patent

active

054948169

ABSTRACT:
DNA molecules encoding a modified tryptophan synthase beta subunit are disclosed. When expressed in a recombinant host microorganism, these polypeptide analogs enable significant levels of intracellular indole production and accumulation. In the presence of an aromatic dioxygenase enzyme, the indole so produced can be converted to indoxyl, which upon exposure to air oxidizes to indigo.

REFERENCES:
Yanofsky et al., "The effects of deletions, point mutations, reversions and suppressor mutations on the two components of the tryptophan synthetase of Escherichia coli," P.N.A.S. 45:1016-1026 (1959).
Bang et al., "Production of L-Tryptophan by Escherichia coli Cells", Biotech & Bioengineering vol. 25, pp. 999-1011 (1993).
Cotton et al., "Tryptophan Synthetase .beta..sub.2 subunit," Journal of Biological Chemistry, vol. 247, No. 6, pp. 1883-1891, (1972).

Affiliated with

Also associated with

Rating

Enhanced indole biosynthesis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enhanced indole biosynthesis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enhanced indole biosynthesis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1678462