Endoglucanases and cellulase preparations containing the same

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S004000, C435S006120, C435S069100, C435S183000, C435S192000, C435S252300, C435S320100, C510S114000, C510S392000, C510S515000, C536S023200, C536S023700

Reexamination Certificate

active

06921655

ABSTRACT:
It is an object of the present invention to provide enzymes that have high endoglucanase activity and yet exhibit high activity even under alkaline conditions, and genes encoding the same. The enzyme according to the invention has the following properties: a) exhibiting endoglucanase activity; and b) capable of completely removing fuzz from regenerated cellulose fabrics at a concentration of 1 mg of the protein/L or below. The enzyme of the invention having endoglucanase activity is a protein comprising the amino acid sequence as shown in SEQ ID NO: 1, 3, 5, 7, 9 or 11; a modified protein thereof exhibiting endoglucanase activity; or a homologue of the protein or the modified protein.

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Saloheimo Anu et al., “A novel, small endoglucanase gene, egI5, fromTrichoderma reeseiisolated by expression in yeast,” Molecular Microbiology (1994), vol. 13, No. 2, pp. 219-228.
Theberge, M. et al.: “Purification and Characterization of an Endoglucanase fromStreptomyces lividans66 and DNA Sequence of the Gene” in Applied and Environmental Microbiology, Washington, DC., US, vol. 58, No. 3, Mar. 1992, pp. 815-820, XP002102200, ISSN: 0099-2240.
Moriya Tatsuki et al.: “Molecular cloning of endo-beta-D-1,4-glucanase genes, rce1, rce2, and rce3, fromRhizopus oryzae.” in Journal of Bacteriology, vol. 185, No. 5 , Mar. 2003 pp. 1749-1756, XP002269183, ISN: 0021-9193.

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