Element-coded affinity tags

Chemistry: analytical and immunological testing – Involving producing or treating antigen or hapten – Producing labeled antigens

Reexamination Certificate

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Details

C436S081000, C436S082000, C436S096000, C436S173000, C435S006120, C435S007100, C435S007500, C435S007920, C435S810000, C435S975000, C435S973000, C530S405000, C530S412000, C540S474000

Reexamination Certificate

active

10835533

ABSTRACT:
The present invention provides compositions and methods for detecting, analyzing, and identifying biomolecules. More particularly, the invention provides Element Coded Affinity Tags comprising a metal chelate and a metal ion and methods of using the tags to detect, analyze, and identify biomolecules including polypeptides, nucleic acids, lipids, and polysaccharides.

REFERENCES:
patent: 5435990 (1995-07-01), Cheng et al.
patent: WO 00/11208 (2000-03-01), None
Aebersold, et al.; “Mass Spectrometry-based Proteomics;”Nature; Mar. 2003; pp. 198-207; vol. 422.
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Gygi, et al.; “Proteome Analysis of Low-Abundance Proteins Using Multidimensional Chromatography and Isotope-Coded Affinity Tags;”Journal of Proteome Research; Jan. 18, 2002; pp. 47-54; vol. 1; American Chemical Society.
Gygi, et al.; “Quantitative Analysis of Complex Protein Mixtures Using Isotope-coded Affinity Tags;”Nature Biotechnology; Oct. 1999; pp. 994-999; vol. 17.
Patterson, et al.; “Proteomics: The First Decade and Beyond;”Nature Genetics Supplement; Mar. 2003; pp. 311-323; vol. 33.
Ranish, et al.; “The Study of Macromolecular Complexes by Quantitative Proteomics;”Nature Genetics; Mar. 2003; pp. 349-355; vol. 33.
Tao, et al.; “Advances in Quantitative Proteomics Via Stable Isotope Tagging and Mass Spectrometry;”Current Opinion in Biotechnology; 2003; pp. 110-118; vol. 14.
Zhou, et al.; “Quantitative Proteome Analysis by Solid-Phase Isotope Tagging and Mass Spectrometry;”Nature Biotechnology; May 2002; pp. 512-515; vol. 20.
Kramerov, A.A., et al., “Tissue Localization and Secretion of Mucin-D inDrosophila melanogaster,” Russian Journal of Developmental Biology(1997) 28(4): 227-234.

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