Electrochemical determination of orthophosphoric monoester phosp

Details Electrochemical determination of orthophosphoric monoester phosp Electrochemical determination of orthophosphoric monoester phosp

1982-08-11

1983-09-27

Kaplan, G. L.

Chemistry: electrical and wave energy

Processes and products

435 21, G01N 2756, C12Q 142

Patent

active

044067518

ABSTRACT:
A method is disclosed for the measurement of acid and alkaline phosphatase activity, whereby an aromatic phosphate ester substrate containing a nitro group is reacted with acid or alkaline phosphatase under appropriate reaction conditions and the hydrolytic process is monitored at electrodes which measure the current produced by the reduction of the nitro groups of the product and/or substrate. Alternatively, an aromatic phosphate ester substrate containing an amino group is reacted with acid or alkaline phosphatase under appropriate reaction conditions with the hydrolytic process being monitored at solid electrodes which measure the current produced by the oxidation of the amino groups of the product and/or substrate.

REFERENCES:
patent: 3896008 (1975-07-01), Keyes
patent: 4059490 (1977-11-01), Axcell et al.

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