Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Patent
1998-04-06
1999-06-22
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
435415, 435418, 435419, 435426, 435468, 435470, 435430, 435431, 800278, 800293, 800312, A01H 500, A01H 104, C12N 504, C12N 1582
Patent
active
059144513
ABSTRACT:
A transformation protocol is described for the particle mediated transformation of soybean. This protocol is based on gene delivery into the growing meristem of a soybean embryo. Prior transformation protocols based on meristematic gene delivery in soybean did not depend on selection due to difficulties in using selection agents in differentiated tissue in soybean. It has been found that a post bombardment culture with glyphosate selection can dramatically increase the efficiency of such a transformation protocol.
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Barwale et al, Planta, vol. 167, pp. 473-481, 1986.
Christou et al., "Stable Transformation of Soybean Callus by DNA-Coated Gold Particles", Plant Physiology 87:671-674 (1988).
Pang et al., "An Improved Green Fluorescent Protein Gene as a Vital Marker in Plants", Plant Physiology 112:893-900 (1996).
Connor-Ward Dannette
Emler Carl
Hinchee Maud A. W.
Julson Lori A.
Martinell Brian
Fox David T.
Mehta Ashwin D.
Monsanto Company
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