E2 subunit vaccine comprising recombinant pestivirus E2 protein

Details E2 subunit vaccine comprising recombinant pestivirus E2 protein E2 subunit vaccine comprising recombinant pestivirus E2 protein

2005-07-19

2005-07-19

Housel, James (Department: 1648)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Virus or component thereof

C424S220100

Reexamination Certificate

active

06919085

ABSTRACT:
The invention relates to a method of increasing protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1×105to 5×106cells/ml with an m.o.i of <0.01. The invention also provides a method to produce recombinant pestivirus E2 or Em9protein or fragments thereof in insect cell culture characterized by a final concentration of the protein fragments in the growth medium at harvest of at least 100 μg/ml. The invention also provides a method of producing recombinant FSH, α-units and/or β-units, and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 μ/ml.

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