Dyed microspheres for characterization of photochemical...

Chemistry: analytical and immunological testing – Optical result – With fluorescence or luminescence

Reexamination Certificate

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C436S546000, C436S800000, C436S805000, C435S007500, C435S968000, C252S588000

Reexamination Certificate

active

07842512

ABSTRACT:
A method for photochemical reactor characterization includes an application of using dyed microspheres exposed to UV irradiation under a collimated-beam system. Particle specific fluorescence intensity measurements are conducted using samples form the collimated beam and flow-through reactor results using flow cytometry. A numerical model may be used to simulate the behavior of the reactor system to provide a particle-tracking algorithm to interrogate the flow and intensity field simulations for purposes of developing a particle specific estimate of the dose delivery. A method for measuring UV dose distribution delivery in photochemical reactors is provided that includes introducing microspheres labeled with a photochemically-active compound in a UV reactor. The labeled microspheres are harvested downstream of the irradiated zone of a UV reactor and exposed to UV irradiation under a collimated beam of UV irradiation. The method further includes quantifying a UV dose-response behavior, conducting fluorescence intensity measurement on the labeled microspheres from the UV reactor, and developing an estimate of a dose distribution delivered by a UV reactor based on the numerical deconvolution of the sum of the UV dose response behavior and fluorescent intensity of exposed microspheres.

REFERENCES:
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“Development of a Nucleoside Analog UV Light Sensor,” by Shiyue Fang et al.; Nucleosides, Nucleotides & Nucleic Acids; vol. 22, Nos. 5-8; pp. 703-705; 2003.
“Dyed Microspheres for Quantification of UV Dose Distributions: Photochemical Reactor Characterization by Lagrangian Actinometry,” by Ernest R. Blatchely III et al.; 27 pages.
Shiyue Fang, Yousheng Guan, Ernest R. Bletchley III, Lian-Shin Lin, Cheng Yue Shen, and Donald E. Bergstrom, entitled “Development of a Nucleoside Analog UV Light Sensor,” Nucleosides, Nucleotides & Nucleic Acids, vol. 22, Nos. 5-8, pp. 703-705, 2003.
Shiyue Fang, Yousheng Guan, Ernest R. Bletchley III, Lian-Shin Lin, Cheng Yue Shen, and Donald E. Bergstrom, entitled “Development of a Nucleoside Analog UV Light Sensor,” Published in Nucleosides, Nucleotides & Nucleic Acids, vol. 22, Issue 5-8; Oct. 8, 2003; pp. 703-705; on-line Article.
W.A. Anderson, L. Zhang, S.A. Andrews, J.R. Bolton, entitled “A Technique for Direct Measurement of UV Fluence Distribution,” AWWA Water Quality Technology Conference, Proceedings, Nov. 2-6, 2003.
Zuzana Bohrerova, Gil Bohrer, S. Mohan Mohanraj, Joel Ducoste, and Karl G. Linden, entitled “Experimental Measurements of Fluence Distribution in a UV Reactor Using Fluorescent Microspheres,” Environmental Science & Technology, vol. 39, No. 22, Oct. 7, 2005, pp. 8925-8930.
Ernest R. Blatchley, III, et al., “Dyed Microspheres for Quantification of UV Dose Distributions: Photochemical Reactor Characterization by Lagrangian Actinometry,” Journal of Environmental Engineering, Nov. 2006, 1390-1403, vol. 132, No. 11.

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