Dry isomerase activation

Details Dry isomerase activation Dry isomerase activation

1975-03-20

1977-05-31

Tanenholtz, Alvin E.

Textiles: fiber preparation

195 62, 195 63, 195 65, 195 66R, 195 68, 195114, C12D 1300

Patent

active

040267644

ABSTRACT:
The isomerase activity of a dry isomerase preparation (e.g., glucose isomerase) is enhanced by initially pretreating the isomerase in an aqueous solution which contains an activating amount of a metal ion activator and/or a thiol generating reducing agent and/or a monosaccharide which the isomerase is capable of isomerizing. Pretreatment temperature and time periods are most suitably conducted in such a manner so as to minimize isomerase deactivation. The pretreatment increases total isomerase activity and thereby renders it more effective for use in an isomerization process (e.g., glucose isomerization).

REFERENCES:
patent: 3623953 (1971-11-01), Cotter et al.
patent: 3694314 (1972-09-01), Lloyd et al.
patent: 3821082 (1974-06-01), Lamm et al.
Danno et al. "Studies on D-Glucose-Isomerizing Activity of D-Xylose-Grown Cells from Bacillus Coagulans, Strain HN-68 Part II, Purification and Properties of D-Glucose-Isomerizing Enzyme," Agr. Biol. Chem., vol. 31, No. 3 pp. 284-292 (1967).
Tsumura et al., "Enzymatic Conversion of D-Glucose to D-Fructose Part IX. Dehydration and Preservation of the Cell as Enzyme Source," Agr. Biol. Chem., vol. 31, No. 8, pp. 908-910 (1967).
Danno et al., "Studies on D-Glucose-Isomerizing Enzyme from Bacillus Coagulans, Strain HN-68 Part VI, The Role of Metal Ions on the Isomerization of D-Glucose and D-Xylose by the Enzyme", Agr. Biol. Chem., vol. 35, No. 7, pp. 997-1006 (1971).
Tsumura et al., "Enzymatic Conversion of D-Glucose to D-Fructose Part VIII, Propagation of Streptomyces Phaechromogenus in the Presence of Cobaltous Ion", Agr. Biol. Chem., vol. 31, No. 8, pp. 902-907 (1967).

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