Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1986-07-31
1989-11-21
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435 68, 435 70, 435 91, 435255, 4351723, 435320, 536 27, 930 11, 930 29, 930 37, 930 38, 930 56, 930 60, 930 69, C12N 100, C12N 1500, C12P 200, C12P 1734
Patent
active
048822825
ABSTRACT:
A chemically-synthesized oligonucleotide composing a portion of the nucleotide sequence of the human IL-2 is employed as a probe to isolate the gene coding for human IL-2. The human IL-2 gene is selected from a cDNA library prepared from RNA produced by mitogen-stimulated Jurkat cells. Double-stranded cDNA is prepared from polyadenylated RNA extracted from bovine cells thought to produce interleukin-2. Such cDNA is inserted within a plasmid vector and the recombinant plasmid employed to transform hosts. Plasmid DNA, prepared from pools of the transformed hosts, is hybridized with a probe composed of a large portion of the coding sequence of the human IL-2 gene. Pools of host cells that provide signal to the human cDNA probe are identified, subdivided, and rescreened until a single positive colony is identified. Bovine plasmid cDNA is prepared from this colony, and the bIL-2 gene is sequenced. The plasmid DNA is employed to express recombinant bovine IL-2 in yeast and bacterial expression systems, with the expressed bovine IL-2 purified to homogeneity by one or more reverse phase high-performance liquid chromatography procedures.
REFERENCES:
patent: 4738327 (1988-04-01), Taniguchi et al.
Miller-Edge et al. (1984) Vetinary Immunology and Immunopathology 4: 119-30.
Morgan and Ruscetti, "Selective In Vitro Growth of T Lymphocytes from Normal Human Bone Marrows," Science 193:1007 (1976).
Ruscetti, et al., "Functional and Morphologic Characterization of Human T Cells Continuously Grown In Vitro," J. Immunol. 119:131 (1977).
Gillis & Smith, "Long Term Culture of Tumor-Specific Cytotoxic T Cells," Nature 268:154 (1977).
Farrar, et al., "Biochemical Relationship of Thymocyte Mitogenic Factor and Factors Enhancing Humoral and Cell-Mediated Immune Responses," J. Immunol. 121:1353 (1978).
Gillis, et al., "T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for Activity," J. Immunol. 120:2027 (1978).
Gillis, et al., "Biochemical Characterization of Lymphocyte Regulatory Molecules," J. Immunol. 124:1954 (1980).
Gillis, et al., "Biochemical and Biologic Characterization of Lumphocyte Regulatory Molecules," J. Exp. Med. 150:849 (1979).
Watson, et al., "Biochemical and Biological Characterization of Lumphocyte Regulatory Molecules," J. Exp. Med. 150:1510 (1979).
Watson, "Continuous Proliferation of Murine Antigen-Specific Helper T Lumphocytes in Culture," J. Exp. Med. 150:1510 (1979).
Baker and Knoblock, "Bovine Costimulator. I. Production Kinetics, Partial Purification, and Quantification in Serum-Free Iscove's Medium," II. Generation and Maintenance of a Bovine Costimulator-Dependent Bovine Lymphoblastoid Cell Line, Vet. Immuno. Immuhopath. 3:365 (1982), and Vet. Immunol. Immunopath. 3:381 (1982).
Splitter and Miller-Edge, "Interleukin 2 (IL-2) Assays Compared By Measuring Bovine IL-2," Fed. Proc. 42:447 (1983).
Ceretti, et al., "Cloning, Sequence, and Expression of Bovine Interleukin 2" Proc. Natl. Acad. Sci. 83:3223 (1986).
Reeves, et al., "Molecular Cloning of a Functional Bovine Interleukin 2 cDNA," Proc. Natl. Acad. Sci. 83:3228 (1986).
Anderson Dirk M.
Baker Paul E.
Cantrell Michael A.
Cerretti Douglas P.
Cosman David J.
Hallquist Scott G.
Immunex Corporation
Nagae Jerald
Seidman S.
Wight Christopher L.
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