Probe for electrofusion, electroporation, or like procedure

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

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Details

435173, 435289, 204242, 204272, C12M 100

Patent

active

048822817

ABSTRACT:
A probe and method for treating samples in a well tray. The probe is specially configured to engage the well, and when so engaged, to provide an electrode structure within the well. The probe comprises an outer electrode in the form of a metal ring having a diameter just slightly less than the inner diameter of the well, a center electrode in the form of a metal rod, and a non-conductive (typically clear plastic) cover that extends over the well and holds the electrodes coaxial with respect to each other and with respect to the well. A metal pin is rigidly connected to the ring and extends parallel to the ring axis. The rod and pin pass through the cover so that their upper ends are accessible for electrical connections. Electrical connections are made to the portions of the rod and the pin located above the cover. As a safety measure, the electrical connections are preferably hidden under a cap that threadingly engages the top portion of the rod and overlies the cover.

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U. Zimmermann and J. Vienken, Electric Field-Induced Cell-to-Cell Fusion, J. Membrane Biol. 67, 165-182 (1982).

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