Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2007-05-15
2011-10-18
Swartz, Rodney P. (Department: 1645)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023100, C435S041000, C435S069100, C435S230000, C435S320100
Reexamination Certificate
active
08039604
ABSTRACT:
The invention consists in a nucleotide sequence having the size of (2646) bp, wherein the order of nucleotides is identical to the order of the nucleotide sequence encoding penicillin acylase fromAchromobactersp. CCM 4824 (formerlyComamonas testosteroniCCM 4824), eventually of the fragments of this sequence having the length of at least 150 nucleotides. The sequence can be used in the formation of a DNA construct, eventually the construct having at least one regulatory sequence regulating the expression of the gene and the production of a polypeptide with the penicillin acylase activity. The sequence can form part of a recombinant expression vector, which consists of the above-mentioned construct, promoter, translational start signal, translational and transcriptional stop signal. Further, the invention concerns a recombinant host cell, containing the nucleic acid construct carried by the vector or integrated into the cell chromosome, and theE. coliBL21 strain containing said sequence of the nucleotides encoding the penicillin acylase carried in the pKXIP1, the pKLP3 or the pKLP6 plasmid.
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Anupama Datla
Becka Stanislav
Hollerova Lenka
Kyslik Pavel
Marsalek Jaroslav
Fermenta Biotech Limited
Law Offices of Albert Wai-Kit Chan PLLC
Mikrobiologicky UTSAV AV CR
Swartz Rodney P.
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