DNA encoding spo-rel polypeptides

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4352523, 4353201, 435325, 536 237, C12P 2106, C12N 120, C12N 1500, C07H 2104

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active

059898640

ABSTRACT:
Spo-rel polypeptides and DNA (RNA) encoding such spo-rel and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such spo-rel for the treatment of infection, particularly bacterial infections. Antagonists against such spo-rel and their use as a therapeutic to treat infections, particularly bacterial infections are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to the presence of spo-rel nucleic acid sequences and the polypeptides in a host. Also disclosed are diagnostic assays for detecting polynucleotides encoding the relA/spoT gene family and for detecting the polypeptide in a host.

REFERENCES:
patent: 5175101 (1992-12-01), Gotz et al.
Gentry, et. al., "Mutational analysis of the Escherichia coli spoT gene identifies distinct but overlapping regions involved in ppGpp synthesis and degradation." Molecular Microbiology, vol. 19, No. 6, pp. 1373-1384, (1996).
Xiao, et. al., "Residual Guanosine 3",5"-Bispyrophosphate Synthetic Activity of relA Null Mutants can Be Eliminated by spoY Null Mutations." The Journal of Biological Chemintry, vol. 266, No. 9, pp. 5980-5990, Issue of Mar. 25, (1991).
Mechold, et. al., "Functional Analysis of relA/spoT Gene Homolog from Streptococcus equisimilis." Journal of Bacteriology, vol. 178, No. 5, pp. 1401-1411. Mar. (1996).
Mechold, et. al., "Genetic organization of the streptokionase region of the Streptococcus equisimilis H46A chromosome." Mol. Gen. Genet, vol. 241, pp. 129-140, (1993).
Fujimura, et. al., "Increase of methicillin Resistance in Staphylococcus aureus caused by deletion of a gene Whose Product is Homologous to Lytic Enzymes." Journal of Bacteriology, vol. 179, No. 20, pp. 6294-6301, Oct. (1997).
Metzger, et. al., Protein Sequences Encoded by the relA and spoT genes of Escherichia coli Are Interrelated. The Journal of Biological Chemistry, Communication, vol. 264, No. 16, pp. 9122-9125, Issue of Jun. 5, (1989).
Lazar et al (Molecular & Cellular Biology vol. 8, No. 3, Mar. 1988 pp. 1247-1252).
Burgess et al (J. of Cell Biology vol. 111, Nov. 1990, pp. 2129-2138).
Salgaller et al (Cancer Immunol. Immunother. vol. 39, 1994, pp. 105-116).
Schendel et al (Biochemistry vol. 28, 1989, pp. 2459-2471).

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