DNA encoding enzyme recombinant DNA and enzyme transformant, and

Details DNA encoding enzyme recombinant DNA and enzyme transformant, and DNA encoding enzyme recombinant DNA and enzyme transformant, and

1996-09-16

1999-02-16

Lau, Kawai

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435101, 435200, 4353201, 4352523, 536 232, C12P 1912, C12N 924, C12N 121, C07H 2104

Patent

active

058719776

ABSTRACT:
A DNA encoding an enzyme, which forms non-reducing saccharides having trehalose structure as an end unit from amylaceous saccharides having a degree of glucose polymerization of 3 or higher, enables an industrial-scale production of a recombinant enzyme with such enzyme activity. Non-reducing saccharides obtainable by the recombinant enzyme can be used in a variety of food products, cosmetics, pharmaceuticals and feeds because of their substantial non-reducibility, mild and high-quality sweetness, adequate viscosity, and moisture-retaining ability.

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patent: 5455168 (1995-10-01), Maruta et al.
patent: 5484714 (1996-01-01), Tsuchida et al.
U.K. Laemmli, "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4", Nature, vol. 227, pp. 680-685, Aug. 15, 1970.
Sambrook et al, Molecular Cloning, A Laboratory Manual, Second Edition, Cold Springs Harbor Press, 1989.
E.M. Southern, "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis", J. Mol. Biol., vol. 98, pp. 503-517, 1975.

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