DNA encoding cysteinyl tRNA synthetase from Staphylococcus aureu

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

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435 6, 4353201, 4352523, 43525411, 435325, 536 232, C12N 900, C12N 1500, C12N 120, C07H 2104

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057767491

ABSTRACT:
The invention provides tRNA synthetase polypeptides and DNA (RNA) encoding tRNA synthetase polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing tRNA synthetase polypeptide for the protection against infection, particularly bacterial infections.

REFERENCES:
Kron, et al., "An immunodominant antigen of Brugia malayi is an asparaginyl-tRNA synthetase", FEBS Letters, 374 pp. 122-124 (1995).
Chalker, et al., "Analysis and toxic overexpression in Escherichia coli of a staphylococcal gene encoding isoleucyl-tRNA synthetase", Gene, 141, pp. 103-108 (1994).
Nilsen, et al., "Cloning and characterization of a potentially protective antigen in lymphatic filariasis", Proc. Natl. Acad. Sci. USA, 85, pp. 3604-3607 (1988).
Fleischmann, et al., "Whole-Genome Sequencing and Assembly of Haemophilus Influenzae Rd", Science, 269, pp. 496-512 (1995) and EMBL Sequence Database, Accession No.: HI32713.
Seror, et al., "A mutant cysteinyl-tRNA synthetase affecting timing of chromosomal replication initiation in B. subtilis and conferring resistance to a protein kinase C inhibitor", The EMBO Journal, 13, No. 10 pp. 2472-2480 (1994).
Gagnon et al. (1994) Clustering and Co-transcription of the Bacillus subtilis Genes Encoding the Aminoacyl-tRNA Synthetases Specific for Glutamate and for Cysteine and the First Enzyme for Cysteine Biosynthesis. J. Biol. Chem. 269(10):7473-7482.

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