Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1985-12-06
1989-10-17
Hazel, Blondel
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435 68, 435320, 536 27, 424 88, 530806, 530350, C12N 120, C12N 1500, C07H 2104
Patent
active
048747050
ABSTRACT:
A genomic DNA molecule having the nucleic acid sequence set forth in FIG. 1 and encoding an antigenic protein derived from Eimeria tenella has been isolated. The protein has a molecular weight of about 25,000 daltons and is composed of two polypeptides joined by a disulfide bond. One of the polypeptides is characterized by a molecular weight of about 17,000 daltons and by a blocked N-terminal amino acid and having the amino acid sequence set forth in FIG. 1. The other polypeptide is characterized by a molecular weight of about 8,000 daltons and has the amino acid sequence set forth in FIG. 1.
A cDNA molecule encoding the 25,000 dalton polypeptide with a continuous amino acid sequence has been inserted into expression vectors capable of expressing the 25,000 dalton polypeptide directly or as a fused polypeptide. The polypeptides produced are used in vaccines to immunize chickens against infection by Eimeria tenella.
REFERENCES:
patent: 4639372 (1987-01-01), Murray et al.
patent: 4650676 (1987-03-01), Schenkel et al.
patent: 4710377 (1987-12-01), Schenkel et al.
Andrews William H.
Brothers Virginia M.
Files James G.
Gore Thomas C.
Kuhn Irene
Hazel Blondel
Solvay & Cie S.A.
White John P.
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