Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...
Reexamination Certificate
2003-09-25
2008-10-07
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing heterocyclic carbon compound having only o, n, s,...
C435S122000, C435S006120, C435S069100, C435S320100, C435S252300, C435S254900, C435S190000, C536S023200
Reexamination Certificate
active
07432089
ABSTRACT:
The present invention relates to a DNA encoding a novel flavin adenine dinucleotide (FAD)-dependent D-erythronate 4-phosphate (EN4P) dehydrogenase originated fromSinorhizobium meliloti, which is involved in vitamin B6 biosynthesis, and a recombinant microorganism transformed with a vector having the DNA. It also relates to a process for production of vitamin B6 by using the recombinant microorganism. “Vitamin B6” as used in this invention includes pyridoxol (PN), pyridoxal and pyridoxamine. Vitamin B6 is a vitamin indispensable to human beings or other animals, and is used as a raw material of medicines or as feed additives.
REFERENCES:
patent: 2005/0164335 (2005-07-01), Yocum et al.
Tazoe et al. (Biosynthesis of vitamin B6 in Rhizobium: in vitro synthesis of pyridoxine from 1-deoxy-D-xylulose and 4-hydroxy-L-threonine, Biosci Biotechnol Biochem. Apr. 2002;66(4):934-6).
Capela, D. et al. “Analysis of the Chromosome Sequence of the Legume SymbiontSinorhizobium melilotiStrain 1021,”Proc. Natl. Acad. Sci., v. 98, No. 17, pp. 9877-9882 (2001).
Capela, D. et al. Database SWALL Database Abstract No. XP-002266688 (2001).
Pease, A.J. et al. “Positive Growth Rate-Dependent Regulation of the pdxA, ksgA, and pdxB Genes ofEscherichia coliK-12,”J. Bacteriol., v. 184, No. 5, pp. 1359-1369 (2002).
EMBL- EBI Database Abstract No. XP-002266689 (2002).
Hoshino Tatsuo
Ichikawa Keiko
Tazoe Masaaki
Bryan Cave LLP
Chowdhury Iqbal H
DSM IP Assets B.V.
Prouty Rebecca E.
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