Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification
Patent
1997-06-16
2000-07-04
Achutamurthy, Ponnathapu
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
435441, 435445, 435447, 435325, 435195, 435106, 4353201, 4352523, 43525233, 43525411, 536 232, C12N 1500, C12N 914, C12N 1555, C12N 510
Patent
active
060837527
ABSTRACT:
A DNA fragment coding for a decarbamylase protein improved in thermostability as the result of replacement of at least one base of a DNA fragment coding for a decarbamylase protein derived from a microorganism with another base and the resultant replacement of at least one of the corresponding amino acids, and its production process; a vector containing the DNA fragment; a transformant obtained by transformation with the vector; as well as a decarbamylase improved in thermostability and its production process. Also disclosed is a process for producing a D-.alpha.-amino acid, which comprises converting an N-carbamoyl-D-.alpha.-amino acid into the corresponding D-.alpha.-amino acid in an aqueous medium by the action of a decarbamylase having a thermostable temperature of 65.degree. C. or higher; and collecting the D-.alpha.-amino acid produced.
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Ikenaka Yasuhiro
Nanba Hirokazu
Takahashi Satomi
Takano Masayuki
Yajima Kazuyoshi
Achutamurthy Ponnathapu
Kanegafuchi Kagaku Kogyo & Kabushiki Kaisha
Mayhew Bradley S.
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