DNA base sequence, method for preparing a recombinant plasmid in

Details DNA base sequence, method for preparing a recombinant plasmid in DNA base sequence, method for preparing a recombinant plasmid in

1988-04-08

1989-04-25

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 68, 435 91, 4353171, 435320, 435243, 43525231, 43525233, 935 29, 935 38, 935 74, 536 27, C12N 1500, C12N 120, C12N 700, C12P 2100

Patent

active

048247820

ABSTRACT:
This invention relates to a DNA base sequence, capable of increasing the amount of protein secreted by microorganisms, and its derivative sequences; a recombinant plasmid including the whole or a part of said DNA base sequence; a method for preparing the recombinant plasmid in which, when microorganisms having introduced thereinto a recombinant DNA including the desired DNA base sequence are to be separated in the process of cloning, suitable transformants can be efficiently selected by taking the amount of protein secreted out of the cells and particularly the activity of an enzyme protein as an index; and a method of microbial breeding which comprises introducing the recombinant plasmid into a microorganism to increase the amount of protein secreted by the microorganism.

REFERENCES:
Chang et al. (1982) NSC Symp. Ser. 4:254-262, abst (CA100 46295).
Palva et al. (1981) Gene 15: 43-51.
Proc. Nat'l Acad. Sci. USA 74, (1977), pp. 560-564.
J. Mo. Biol., 3, (1961), pp. 208 to 218.
Mol. Gen. Genet., 168, (1979), pp. 111 to 115.
Nucleic Acid Res., 7, (1979), pp. 1513 to 1523.
J. Biochem., 45, (1958), pp. 185-194.

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