Direct molecular cloning of primer extended DNA containing an al

Details Direct molecular cloning of primer extended DNA containing an al Direct molecular cloning of primer extended DNA containing an al

1993-09-08

1995-06-20

Parr, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 914, 435 9151, 43525233, C12P 1934, C12N 120

Patent

active

054260396

ABSTRACT:
This invention relates to a method of cloning DNA produced by primer extension including PCR amplified, reverse transcriptase-generated or primer extended synthetic DNA. Specifically, it relates to a method in which alkane diol residue containing oligonucleotide primers are incorporated into DNA by primer extension followed by direct cloning of the target DNA. Following transformation, the host excises the alkane diol residue with its endogenous DNA repair machinery.

REFERENCES:
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Ugozzoli, L. et al., "Detection of Specific Alleles by Using Allele-Specific Primer Extension Followed by Capture on Solid Support," GATA, 9(4):107-112, (1992).
Newton, C. R., et al., "The Production of PCR Products with 5' Single-Stranded Tails Using Primers that Incorporate Novel Phosphoramidite Intermediates," Nucleic Acids Research, vol. 21, No. 5, pp. 1155-1162, (1993).
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