Diagnostic tests for inflammatory bowel diseases

Details Diagnostic tests for inflammatory bowel diseases Diagnostic tests for inflammatory bowel diseases

2011-07-05

2011-07-05

Weber, Jon P (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

Reexamination Certificate

active

07972807

ABSTRACT:
Disclosed are diagnostic tests helpful in indicating presence of an inflammatory bowel disease (IBD) in a human patient. In one embodiment, the test comprises obtaining a sample of mucosal tissue from the ileum or sigmoid colon of the patient; evaluating sample quality by testing for cadaverine and continuing the diagnostic test if the sample has no detectable cadaverine; testing the cadaverine negative sample for N-acetylated spermine; and correlating a detectable level of N-acetylated spermine in the sample with presence of IBD in the patient. Another, less invasive, method disclosed includes a diagnostic test comprising isolating mononuclear leukocytes from the patient's blood; testing the isolated mononuclear leukocytes for level of spermidine; and correlating a level of spermidine higher than that in mononuclear leukocytes of normal subjects as indicative of an inflammatory bowel disease in the patient.

REFERENCES:
Villanueva et al. “Chromatography, flow injection analysis and electrophoresis in computer-assisted comparative biochemistry: its application and possibilities in clinical research”, J of Chromatography, 1988, 440, 261-273.
Obayashi et al. “Polyamine metabolism in colonic mucosa from patients with ulcerative colitis”, The American Journal of Gastroenoterology, 1992, 87(6):736-740.
Weiss et al. “Intracellular polyamine levels of intestinal epithelial cells in inflammatory bowel disease”, Inflamm Bowel Dis., 2004, 10(5):529-535.
Macfarlane et al. “Mucosal bacteria in ulcerative colitis”, British Journal of Nutrition, 2005, 93, Suppl. 1:S67-S72.

Affiliated with

Also associated with

Rating

Diagnostic tests for inflammatory bowel diseases does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Diagnostic tests for inflammatory bowel diseases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Diagnostic tests for inflammatory bowel diseases will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2735920