Diagnosis and treatment of chronic lymphocytic leukemia (CLL)

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C536S023100, C536S024310, C536S024330

Reexamination Certificate

active

07846664

ABSTRACT:
The present invention provides diagnostic methods and kits for diagnosis of chronic lymphocytic leukemia (CLL) by determining expression levels of isoforms of cyclic nucleotide phosphodiesterases (PDEs) associated with CLL particularly, PDE7B and/or PDE3B, and a ratio of mRNA expression of PDE7B to PDE3B. The present invention provides that CLL lymphocytes uniformly expressed high levels of PDE7B and low levels of PDE3B relative to those of normal lymphocytes. A method of treatment and a pharmaceutical composition for CLL comprising one or more therapeutic agents capable of modulating expression or activity levels of isoforms of PDEs associated with CLL, and/or reversing the ratio of PDE7B/PDE3B mRNA expression levels are also provided.

REFERENCES:
patent: 6020135 (2000-02-01), Levine et al.
patent: 6818651 (2004-11-01), Weinbrenner et al.
patent: 6849638 (2005-02-01), Stolle et al.
patent: 2004/0018513 (2004-01-01), Downing et al.
Moon et al., 2002, “Inhibition of PDE3B Augments PDE4 Inhibitor-Induced Apoptosis in a Subset of Patients with Chronic Lymphocytic Leukemia,” Clinical Cancer Research, vol. 8, 589-595.

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