Diacylglycerol acyltransferases for alteration of...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S252300, C435S257200, C435S254110, C435S069100, C435S006120, C435S193000, C435S134000, C536S023200

Reexamination Certificate

active

11024544

ABSTRACT:
Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g.,Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated fromY. lipolyticaandMortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.

REFERENCES:
patent: 6100077 (2000-08-01), Sturley et al.
patent: 6344548 (2002-02-01), Farese, Jr. et al.
patent: 6552250 (2003-04-01), Nykiforuk et al.
patent: 2004/0078836 (2004-04-01), Farese, Jr. et al.
patent: 2004/0088759 (2004-05-01), Cahoon et al.
U.S. Appl. No. 10/882,760, filed Jul. 1, 2004, Yadav et al.
Hongyuan Yang et al., Sterol Esterification in Yeast: A Two-Gene Process, Science, vol. 272:1353-1356, 1996.
Heidemarie Mullner et al., Dynamics of neutral lipid storage in yeast, Acta Biochimca Polonica, vol. 51:323-347, 2004.
D. Sorger et al., Triacylglycerol biosynthesis in yeast, Appl. Microbiol. Biotechnol., 61:289-299, 2003.
Line Sandager et al., Storage Lipid Synthesis Is Non-essential in Yeast, J. Biol. Chem., vol. 277(8):6478-6482, 2002.
Anders Dahlqvist et al., Phospholipid:diacylglycerol acyltransferase: An enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants, PNAS, vol. 97(12):6487-6492, 2000.
L. Sandager et al., An acyl-CoA:cholesterol acyltranseferase (ACAT)-related gene is involved in the accumulation of triacylglycerols inSaccharomyces cerevislae, Biochemical Society Transactions, vol. 28(6):700-702, 2000.
Kathryn D. Lardizabal et al., DGAT2 Is a New Diacylglycerol Acyltransferase Gene Family, J. Biol. Chem., vol. 276(42):38862-38869, 2001.
National Center for Biotechnology Information General Identifier No. 41387496, Accession No. AY445635, Jul. 1, 2004, K. Giannoulia et al., Olive DGAT1 CDNA.
National Center for Biotechnology Information General Identifier No. 15099950, Accession No. AF384160, Oct. 16, 2001, S. Cases et al., Cloning of DGAT2, A Second Mammalian Diacylglycerol.
National Center for Biotechnology Information General Identifier No. 17865334, Accession No. NM—053437, Apr. 20, 2005. J. J. Liang et al., Overexpression of Human Diacylglycerol Acyltransferase 1, Acyl-CoA:Cholesterol Acyltransferase 1, or Acyl-CoA:Cholesterol Acyltransferase 2 Stimulates Secretion of Apolipoprotein B-Containing Lipoproteins in MCA-RH7777 Cells.
National Center for Biotechnology Information General Identifier No. 27819635, Accession No. NM—174693, Apr. 23, 2005, C. Kuhn et al., Evidence for Multiple Alleles at the DGAT1 Locus Better Explains a Quantitative Trait Locus With Major Effect on Milk Fat Content in Cattle.
National Center for Biotechnology Information General Identifier No. 21684669, Accession No. AY116586, Dec. 30, 2002, D. Nonneman et al., Linkage Mapping of Porcine DGAT1 to a Region of Chromosome 4 That Contains QTL for Growth and Fatness.
National Center for Biotechnology Information General Identifier No. 33113254, Accession No. AY327327, Nov. 3, 2004, F. Quittnat et al., on the Biogenesis of Lipid Bodies in Ancient Eukaryotes:Synthesis of Triacylglycerols by a Toxoplasma DGAT1-Related Enzyme.
National Center for Biotechnology Information General Identifier No. 33113252, Accession No. AY327326, Nov. 3, 2004, F. Quittnat et al., on the Biogenesis of Lipid Bodies in Ancient Eukaryotes:Synthesis of Triacylglycerols by a Toxoplasma DGAT1-Related Enzyme.
National Center for Biotechnology Information General Identifier No. 10803052, Accession No. AF298815, Oct. 16, 2000, S.-K. Hwang et al., Isolation of Perilla Frutescens Diacylglycerol Acyltransferase CDNA.
National Center for Biotechnology Information General Identifier No. 5579407, Accession No. AF164434, Nov. 30, 1999, C.L. Nykiforuk et al., Isolation and Characterization of a CDNA Encoding a Second Putative Diacylglycerol Acyltransferase From a Microspore-Derived Cell Suspension Culture ofBrassica napusL. CV Jet NEUF.
National Center for Biotechnology Information General Identifier No. 34582301, Accession No. Q876L2, Mar. 15, 2004, R.B. Langkjaer et al., Yeast Genome Duplication was Followed by Asynchronous Differentation of Duplicated Genes.
National Center for Biotechnology Information General Identifier No. 1703371, Accession No. P53629, May 1, 2005, H. Yang et al., Sterol Esterification in Yeast:A Two-Gene Process.
National Center for Biotechnology Information General Identifier No. 1703372, Accession No. Q10269, May 1, 2005, V. Wood et al., The Genome Sequence ofSchizosaccharomyces pombe.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Diacylglycerol acyltransferases for alteration of... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Diacylglycerol acyltransferases for alteration of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Diacylglycerol acyltransferases for alteration of... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3730719

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.