Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1994-12-13
1997-06-10
Lieberman, Paul
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4351723, 4353201, 510392, 510530, 536 232, C12N 944
Patent
active
056374917
ABSTRACT:
A method for the isolation and expression of a gene coding for dextranase of the fungus Penicillium minioluteum. This enzyme of fungal origin is produced by expression at high levels in yeast. For this purpose, a cDNA copy of the mRNA coding for dextranase enzyme of the Penicillium minioluteum fungus was isolated and sequenced. This cDNA was transfered into Pichia pastoris cells. Recombinant yeasts capable of secreting dextranase to the culture medium were obtained thereby. The dextranase enzyme obtained can be used, e.g. in the sugar industry to hydrolyze the dextran in cane juices to increase the sugar production.
Campana Hernan R.
Clark Emilio M.
Cordova Vivian M.
Cremata Alvarez Jose A.
Curbelo Dania M.
Centro de Ingenieria Genetica y Biotecnologia
Fries Kery A.
Lieberman Paul
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