Device and method for rapidly lysing cells or viruses

Details Device and method for rapidly lysing cells or viruses Device and method for rapidly lysing cells or viruses

2011-03-22

2011-03-22

Ford, Allison M. (Department: 1651)

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Cell membrane or cell surface is target

C435S173100

Reexamination Certificate

active

07910343

ABSTRACT:
A cell lysis device for lysing cells or viruses, comprising a cell lysis tube having a sample inlet; a pump connected to the cell lysis tube for transferring a sample into the tube; a sealing unit for reversibly sealing a specific region of the tube; and a laser source for generating a laser is provide. Further, a method of lysing cells or viruses using the cell lysis device is provide. The method comprises introducing a sample containing cells or viruses and optionally magnetic beads to the cell lysis tube through the sample inlet; transferring the sample to a specific region in the cell lysis tube by means of the pump; temporarily sealing the region of the cell lysis tube where the sample is placed with the sealing unit; irradiating the sample with the laser; removing the sealing unit from the cell lysis tube; and discharging the sample from the cell lysis tube by means of the pump.

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