Determination of NAD(P)H or salicylate

Details Determination of NAD(P)H or salicylate Determination of NAD(P)H or salicylate

1981-12-07

1983-11-22

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 14, 435 13, 435 19, 435 22, 435 23, 435 26, 435189, 435805, 435810, C12Q 126, C12Q 132, C12Q 138, C12Q 140, C12Q 114, C12Q 154, C12Q 156, C12N 902

Patent

active

044169833

ABSTRACT:
The present invention provides a process for the determination of NAD(P)H or of salicylate, wherein, in a NAD(P)H-dependent reaction, salicylate is decarboxylated by salicylate hydroxylase and a colored material is formed from the decarboxylation product in the presence of tyrosinase by oxidative coupling with an appropriate colored material component, the colored material formed then being determined photometrically.
The present invention also provides a reagent for the determination of NADH or NADPH, wherein it contains salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer, as well as a reagent for the determination of salicylate, wherein it contains NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.

REFERENCES:
patent: 4168205 (1979-09-01), Danninger et al.
White-Stevens et al., J. Biol. Chem., 247(8), 2358-2370 (1972).
Dawson et al. Methods in Enzymology, vol. II, Academic Press, Inc., New York, 817-828 (1955).

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