Chemistry: analytical and immunological testing – Hemoglobin – myoglobin – or occult blood – Glycosylated hemoglobin
Patent
1997-03-13
2000-12-19
Wallenhorst, Maureen M.
Chemistry: analytical and immunological testing
Hemoglobin, myoglobin, or occult blood
Glycosylated hemoglobin
436 66, 436164, 436172, 436805, 436824, 436518, 436524, 435 71, 435960, G01N 3372
Patent
active
061626457
ABSTRACT:
A glycated hemoglobin assay utilizes a simple procedure for the determination of standardized %GHb in whole blood samples correlated to the Diabetes Control and Complications Trial (DCCT). First, a lysed whole blood sample is incubated with a solid phase that is coupled with boronic acid or similar boronate compound through covalent linkage chemistries known in the art. Next, a labeled antibody to human hemoglobin is added and the resulting signal is directly proportional to the %GHb in the sample. The advantages of measuring %GHb using a single determination include high precision and, since the assay is easily automatable, high throughput. With automation, this assay can also be consolidated with other testing on one analyzer. The method according to the various embodiments of the invention thus eliminates the need for two measurements: one for GHb and another for total hemoglobin (THb).
REFERENCES:
patent: 4269605 (1981-05-01), Dean et al.
patent: 4496722 (1985-01-01), Gallop et al.
patent: 4727036 (1988-02-01), Knowles et al.
patent: 4861728 (1989-08-01), Wagner
patent: 5045234 (1991-09-01), Bonicolini et al.
patent: 5110745 (1992-05-01), Kricka et al.
patent: 5206144 (1993-04-01), Zeuthen et al.
patent: 5242842 (1993-09-01), Sundrehagen
patent: 5284777 (1994-02-01), Rosenthal et al.
patent: 5294336 (1994-03-01), Mizuno et al.
patent: 5348649 (1994-09-01), Mizuno et al.
patent: 5468646 (1995-11-01), Mattingly et al.
patent: 5470759 (1995-11-01), Sugiyama et al.
patent: 5478754 (1995-12-01), Brandt et al.
patent: 5506114 (1996-04-01), Sangha
patent: 5506144 (1996-04-01), Sundrehagen
patent: 5543524 (1996-08-01), Mattingly et al.
patent: 5589393 (1996-12-01), Fiechtner et al.
D.E. Goldstein et al, "Glycated Hemoglobin: Methodologies and Clinical Applications", Clin Chem, vol. 32 (1986), pp. B64-B70.
Adamczyk Janina
Christensen Melissa A.
Lee Evelyn Mok
Westerberg David A.
Yao Haiou H.
Abbott Laboratories
Anderson Regina M.
Wallenhorst Maureen M.
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