Determination of amylase

Details Determination of amylase Determination of amylase

1974-11-15

1976-04-27

Tanenholtz, Alvin E.

Textiles: fiber preparation

1951035C, G01N 3114

Patent

active

039532976

ABSTRACT:
A method for the determination of amylase activity and a useful highly stable color reagent aqueous solution therefore are disclosed. The solution comprises, as a color reagent, an aromatic nitro containing compound such as 3,5-dinitrosalicyic acid, a color stabilizing chelating compound such as ethylenediaminetetraacetic acid, and a hydroxide base. Eliminating the necessity for centrifuging prior to the determination of amylase activity can be achieved by including potassium hydroxide for at least a part of the base in the solution.

REFERENCES:
patent: 3413198 (1968-11-01), Deutsch
patent: 3758384 (1973-09-01), Babson et al.
Searcy et al., The American Journal of Clinical Pathology, Vol. 46, No. 5, pp. 582-586 (1966).

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