Detection reagent for shiga toxin family gene of...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C435S006120, C435S091200

Reexamination Certificate

active

11048794

ABSTRACT:
The present invention provides a combination of oligonucleotides preferable for composing a rapid and specific gene testing reagent for Shiga toxin family gene type 2 (stx) of entero-hemorrhagicEscherichia coli(EHEC). More specifically, the present invention provides a method for detecting stx2 RNA of EHEC by specifically amplifying only stx2 RNA using a primer having a sequence homologous or complementary to a base sequence specific for stx2 gene of EHEC and located at sites free of alterations between genotypes, and an oligonucleotide that binds to a specific site of stx2 RNA.

REFERENCES:
patent: 6080400 (2000-06-01), Williams et al.
patent: 2003/0008305 (2003-01-01), Maruyama et al.
patent: 199 46 296 A 1 (2001-03-01), None
patent: 1 236 806 (2002-09-01), None
patent: 1 375 674 (2004-01-01), None
patent: WO 01/94634 (2001-12-01), None
Buck et al., Design strategies and performance of custom DNA sequencing primers. BioTechniques (1999) 27: 528-536.
Product #1256, Random Primer 24, New England Biolabs Catalog (1998-1999), p. 121.
CD-ROM containing an unbridged electronic version of the article on the “Preparation and Utilization of Isolated and Purified Oligonucleotides” by Dr. Andrew Chin.
Derwent Publications, AN 2004-233274, XP-002357559, JP 2004-081054, Mar. 18, 2004.
CD-ROM containing an unbridged electronic version of the article on the “Preparation and Utilization of Isolated and Purified Oligonucleotides” by Dr. Andrew Chin, Mar. 9, 2002.

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