Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2008-03-18
2008-03-18
Kim, Young J. (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S006120, C435S091200
Reexamination Certificate
active
11048794
ABSTRACT:
The present invention provides a combination of oligonucleotides preferable for composing a rapid and specific gene testing reagent for Shiga toxin family gene type 2 (stx) of entero-hemorrhagicEscherichia coli(EHEC). More specifically, the present invention provides a method for detecting stx2 RNA of EHEC by specifically amplifying only stx2 RNA using a primer having a sequence homologous or complementary to a base sequence specific for stx2 gene of EHEC and located at sites free of alterations between genotypes, and an oligonucleotide that binds to a specific site of stx2 RNA.
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Buck et al., Design strategies and performance of custom DNA sequencing primers. BioTechniques (1999) 27: 528-536.
Product #1256, Random Primer 24, New England Biolabs Catalog (1998-1999), p. 121.
CD-ROM containing an unbridged electronic version of the article on the “Preparation and Utilization of Isolated and Purified Oligonucleotides” by Dr. Andrew Chin.
Derwent Publications, AN 2004-233274, XP-002357559, JP 2004-081054, Mar. 18, 2004.
CD-ROM containing an unbridged electronic version of the article on the “Preparation and Utilization of Isolated and Purified Oligonucleotides” by Dr. Andrew Chin, Mar. 9, 2002.
Horie Ryuichi
Masuda Noriyoshi
Yasukawa Kiyoshi
Kim Young J.
Thomas David C.
Tosoh Corporation
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