Detection of viruses by amplification and hybridization

Details Detection of viruses by amplification and hybridization Detection of viruses by amplification and hybridization

1989-08-15

1993-01-05

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 435810, 436811, 536 2432, 536 2433, 935 78, C12Q 168, C12P 1934, G01N 3300, C07H 1512

Patent

active

051769956

ABSTRACT:
The presence or absence of a nucleic acid sequence associated with one or more related viruses in a sample containing one or more nucleic acids and suspected of containing such sequence can be detected by amplifying the sequence using primers to form extension products as templates and detecting the amplified product if it is present. This may be accomplished by adding a labeled hybridization probe to the amplified product either free in solution or after immobilization on a solid support. Preferably the virus constitutes AIDs viruses and hepadnaviruses.

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