Detection of nucleic acids in cells by thermophilic strand displ

Details Detection of nucleic acids in cells by thermophilic strand displ Detection of nucleic acids in cells by thermophilic strand displ

1995-09-21

1998-03-31

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 5, 435 6, 536 243, C12P 1934, C12Q 168, C07H 2104

Patent

active

057337527

ABSTRACT:
Thermophilic Strand Displacement Amplification (tSDA) for amplification of nucleic acid target sequences in situ in cells in suspension, on slides or in tissues is described. Excellent specimen morphology is preserved, and either DNA targets, RNA targets, or both may be selectively amplified. In situ amplification by tSDA is compatible with immunochemical techniques, so that both amplification of target sequences and immunological staining can be performed on the same specimen.

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patent: 5631147 (1997-05-01), Lohman et al.
Saiki et al, "Primer-directed enyzmatic amplification of DNA polymerase", Science 239:487-491, Jan. 1988.
G.T. Walker, et al. "Strand displacement amplification--an isothermal, in vitro DNA amplification technique" Nucl. Acids Res. 20, 1691-1696 (1992).
G.T. Walker, et al."Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymearse system" Proc. Natl. Acad. Sci, USA 89, 392-396 (1992).
G.J. Nuovo, et al. "Detection of human papillomavirus DNA in formalin-fixed tissues by in situ hybridization after amplification by Polymerase Chain Reaction" Amer. J. Pathol. 139, 847-854 (1991).
G.J. Nuovo, et al. "An improved technique for the in situ detection of DNA after Polymerase Chain Reaction Amplification" Amer. J. Pathol. 139, 1239-1244 (1991).
G.J. Nuovo, et al."In situ localization of PCR-amplified humand and viral cDNAs" PCR Meth. Appl. pp. 117-123 (1992).

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