Detection and amplification of ligands

Details Detection and amplification of ligands Detection and amplification of ligands

2007-09-10

2010-06-08

Shibuya, Mark L (Department: 1641)

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

C436S518000, C435S007100, C435S005000, C435S007900, C435S007200, C435S069100, C349S123000, C349S124000, C349S130000, C349S137000, C536S023400, C536S023500

Reexamination Certificate

active

07732219

ABSTRACT:
A system for the detection of ligands comprising at least one receptor and an amplification mechanism coupled to the receptor wherein an amplified signal is produced as a result of receptor binding a ligand. Examples of suitable amplification mechanisms include antibody-embedded liquid crystalline materials; use of alpha-2-macroglobulin to encage an enzyme, whereby the enzyme is separated from its substrate by an receptor; and a receptor engineered to inhibit the active of site of an enzyme only in the absence of a ligand. Also provided are methods for the automatic detection of ligands.

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