Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-06-28
1998-11-03
Myers, Carla J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 435 915, 435 9151, 536 235, 536 243, 536 2433, 935 6, 935 8, 935 17, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104
Patent
active
058306560
ABSTRACT:
The present invention provides a novel method for detecting the presence, or absence, or reduced level of an mRNA transcript of a novel tumor suppressor gene, hereinafter referred to as the "CATR1 gene". The method comprises isolating mRNA from tissue samples, amplifying the mRNA by reverse transcriptase-PCR using primers specific to a region in the CATR1 gene, and detecting the presence or absence of the amplified product to determine whether CATR1 mRNA is present or absent or present at reduced levels in the tissue sample. Optionally, the CATR1 mRNA when present, is also quantified. The present invention also relates to the primers which are used in the method. The present invention also relates to a segment of the CATR1 gene, hereinafter referred to as the "CATR 1.3 genetic element," which is useful for designing the primers used in the method of detecting CATR1 mRNA. The CATR 1.3 genetic element is also useful for preparing antisense nucleic acid segments which are CATR1 gene specific inhibitors.
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Casto Bruce C.
Chen Jucheng
Li Dawei
Milo, Jr. George E.
Noyes Inge
Myers Carla J.
Shoemaker Debra
The Ohio State Research Foundation
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