Dehydrogenase and a gene encoding the same

Details Dehydrogenase and a gene encoding the same Dehydrogenase and a gene encoding the same

2006-10-03

2006-10-03

Achutamurthy, Ponnathapu (Department: 1652)

Organic compounds -- part of the class 532-570 series

Organic compounds

Unsubstituted hydrocarbyl chain between the ring and the -c-...

C548S950000, C514S211010, C435S179000

Reexamination Certificate

active

07115735

ABSTRACT:
The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties:(1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme;(2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium;(3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and(4) when treated at 30 ° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.

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