Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...
Patent
1996-04-04
1998-08-18
Stucker, Jeffrey
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
435 13, 435325, 435370, 435374, A01N 102
Patent
active
057957118
ABSTRACT:
An artificial liver support system is described herein which comprises cryopreserved hepatocytes having an initial viability of 80-99% and a metabolic activity 50-80% of fresh hepatocytes. Further disclosed are hepatocytes cryopreserved by dispensing hepatocytes into freezing containers, freezing the containers from between minus 50 to minus 90 degrees Celsius, storing the containers in liquid or vapor nitrogen, thawing the cryopreserved hepatocytes when ready for use and removing residual cryoprotectant media.
REFERENCES:
Kasai et al. "Large-Scale Cryopreservation of Isolated Dog Hepatocytes" Cryobiology, vol. 30 (1993), pp. 1-11.
Cain Shawn Paul
Jauregui Hugo O.
Mullon Claudy Jean-Paul
Naik Sharda
Perlman Timothy Jon
Circe Biomedical, Inc.
Stucker Jeffrey
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