Cryopreserved hepatocytes and high viability and metabolic activ

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...

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435 13, 435325, 435370, 435374, A01N 102

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active

057957118

ABSTRACT:
An artificial liver support system is described herein which comprises cryopreserved hepatocytes having an initial viability of 80-99% and a metabolic activity 50-80% of fresh hepatocytes. Further disclosed are hepatocytes cryopreserved by dispensing hepatocytes into freezing containers, freezing the containers from between minus 50 to minus 90 degrees Celsius, storing the containers in liquid or vapor nitrogen, thawing the cryopreserved hepatocytes when ready for use and removing residual cryoprotectant media.

REFERENCES:
Kasai et al. "Large-Scale Cryopreservation of Isolated Dog Hepatocytes" Cryobiology, vol. 30 (1993), pp. 1-11.

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