Cryo-charging specimen holder for electron microscope

Details Cryo-charging specimen holder for electron microscope Cryo-charging specimen holder for electron microscope

2008-03-28

2010-02-09

Nguyen, Kiet T (Department: 2881)

Radiant energy

Inspection of solids or liquids by charged particles

Electron microscope type

Reexamination Certificate

active

07659510

ABSTRACT:
The present invention relates to a cryo-charging specimen holder for the electron microscope, particularly to a cryo-charging specimen holder for the electron microscope to hold various biological materials. The major feature of the invention is to charge the biological specimen and freeze the specimen at low temperature. The ice around the biological sample is also doped, so that after charging the doped ice surrounding the sample has a conductivity level comparable to that of conductor. Therefore, the sample can be embedded by the doped and charged ice obtaining the property of conductor, in order to be observed by the electron microscope.

REFERENCES:
patent: 6875984 (2005-04-01), Kakibayashi et al.
patent: 7413872 (2008-08-01), Frederik et al.
patent: 7420184 (2008-09-01), van de Water et al.
Chao, Chih-Yu; Bio-Molecular Microscopy at Atomic Resolution, Chinese Journal of Physics, Dec. 2007, vol. 45, No. 6-I, pp. 557-578, The Physical Society of the Republic of China.

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