Cross-linked enzyme membrane

Details Cross-linked enzyme membrane Cross-linked enzyme membrane

1979-02-01

1981-02-17

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

435 95, 435137, 435176, 435180, 435190, 435213, 435288, 435280, C12P 1304, C12N 1108, C12M 140, C12N 976

Patent

active

042516315

ABSTRACT:
A cross-linked enzyme membrane is prepared by directly adsorbing enzymes into the pores of a microporous non-fibrous filter membrane made of a silica modified vinylchloride polymer and then cross-linking the enzyme with a bi-functional coupling agent whereby enzyme molecules are cross-linked to each other without chemically bonding the enzyme molecules to the membrane. The cross-linked enzyme membrane is used to carry out enzymatic reactions by passing a solution of substrate for the enzyme through the membrane under a pressure differential.

REFERENCES:
patent: 3705084 (1972-12-01), Reynolds
patent: 3796634 (1974-03-01), Haynes et al.
patent: 3804719 (1974-04-01), Messing
patent: 3977941 (1976-08-01), Vieth et al.
patent: 4102746 (1978-07-01), Goldberg
Silman et al., Papain Membrane on a Collodion Matrix Science, vol. 150, 1965 (pp. 758-760).
Simon et al., Effectiveness of Enzyme--Membrane Filtration Reactors, Biotechnological Applications of Protein and Enzymes, Academic Press, N.Y., 1977 (pp. 169-181).
Zaborsky, O., Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973 (pp. 61-74).

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