Continuous isoelectric separation

Details Continuous isoelectric separation Continuous isoelectric separation

1988-11-23

1992-05-19

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204301, 2041823, 2041826, 2041801, G01N 2726, B01D 5702

Patent

active

051145553

ABSTRACT:
A method and apparatus for the continuous separation of a target protein from a protein mixture containing two or more proteins and at a pH equal to the pI of the target protein is disclosed. The method and apparatus employ a non-ionic, non-electrically conductive porous conduit through which the protein mixture is passed. The conduit is subjected to the influence of an electrical field substantially perpendicular to the flow of fluid therethrough resulting in movement of all charged protein from the conduit lumen. The target protein, which is unaffected by the electric field since it is not charged at a pH equal to its pI, is collected from the conduit outlet in substantially purified form.

REFERENCES:
patent: 2976576 (1961-03-01), Wichterle et al.
patent: 3051640 (1962-08-01), Treaxler
patent: 3240692 (1966-03-01), Donnelly
patent: 3704223 (1972-11-01), Dietzsch et al.

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