Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1996-09-09
1998-05-26
Ketter, James
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4353201, 536 231, 536 253, C12N 1511, C12N 1563, C07H 2100
Patent
active
057563250
ABSTRACT:
The present invention concerns a polynucleotide having both ribonucleotides and deoxyribonucleotides in a first strand and solely deoxyribonucleotides in a second strand; wherein the strands are Watson-Crick paired and are linked by an oligonucleotide so that the polynucleotide has at most a single 3' and a single 5' end. These ends can be ligated so that the polynucleotide is a single continuous circular polymer. The polynucleotide can be used to induce specific alterations in targeted genes.
REFERENCES:
patent: 4503151 (1985-03-01), Paddock
patent: 4873191 (1989-10-01), Wagner et al.
patent: 4882279 (1989-11-01), Cregg
patent: 4950599 (1990-08-01), Bertling et al.
patent: 5013830 (1991-05-01), Ohtsuka et al.
patent: 5589369 (1996-12-01), Seidman et al.
Hug et al. Liposomes for the transformation of eukaryotic cells. Biochimica et aBiophysica Acts vol. 1097, pp. 1-17, 1991.
Potter et al. Enhancer-dependent expression of human kappa immunoglobulin genes intorduced into mouse pre-B lymphocytes by electroporation. Proc. Natl. Acad. Sci. USA vol. 81, pp. 7161-7165, 1984.
Roberts, R.W., & Crothers, D.M., "Stability and Properties of Double and Triple Helices: Dramatic Effects of RNA or DNA Backbone Composition", Science 258:5087,1463-1466 (1992).
Shimizu et al., "Oligo(2'-O-methyl)ribonucleotides--Effective probes for duplex DNA", FEBS Letters 302:2, 155-158 (1992).
Monia et al., "Selective Inhibition of Mutant Ha-ras mRNA Expression by Antisense Oligonucleotides", J. Biol Chem 267:28, 19954-19962 (1992).
Monia et al., "Evaluation of 2'-Modified Oligonucleotides Containing 2'-Deoxy Gaps as Antisense Inhibitors of Gene Expression", J Biol Chem 268:19, 14514-14522 (1993).
Lesnik et al., "Oligodeoxynucleotides Containing 2'-O-Modified Adenosine: Synthesis and Effect on Stability of DNA:RNA Duplexes", Biochemistry 32:7832-7838 (1993).
Shimizu et al., "Effects of 5-Methyl Substitution in 2'-O-Methyloligo-(Pyrimidine)Nucleotides on Triple-Helix Formation", Bio & Med Chem Ltrs 4:8, 1029-1032 (1994).
Taparowski et al., "Activation of the T24 Bladder Carcinoma Transforming Gene is Linked to a Single Amino Acid Change", Nature 300:762 (1982).
Sukamar et al., "Induction of Mammary Carcinomas in Rats by Nitroso-Methylurea Involves Malignant Activation of H-ras-1 Locus by Single Point Mutations", Nature 306:658 (1983).
Caruthers, "Gene Synthesis Machines: DNA Chemistry and Its Uses", Science 230:281-285 (1985).
Thomas and Capecchi, "Site-Directed Mutagenesis by Gene Targeting in Mouse Embryo-Derived Stem Cells", Cell 52:503 (1987).
Moerschell et al., "Transformation of Yeast with Synthetic Oligonucleotides", PNAS USA 85:524-528 (1988).
Brinster et al., "Targeted Correction of a Major Histocompatibility Class II E.sub.a Gene by DNA Microinjected into Mouse Eggs", PNAS USA 86:7087 (1989).
Capecchi, "Altering the Genome by Homologous Recombination", Science 244:1288 (1989).
Perreault et al., "Mixed Deoxyribooligonucleotides with Catalytic Activity", Nature 344:565 (1990).
Scaringe et al., "Chemical Synthesis of Biologically Active Oligonucleotides Using .beta.-Cyanoethyl Protected Ribonucleoside Phosphoraidites", Nuc Acids Res 18:5433-5441 (1990).
Wahls et al., "The Z-DNA Motif d(TG).sub.30 Promotes Reception of Information during Gene Conversion Events while Stimulating Homologous Recombination in Human Cells in Culture", Mol Cell Biol 10:785-793 (1990).
Yang et al., "Mixed DNA/RNA Polymers Are Cleaved by the Hammerhead Ribozyme", Biochemistry 29:11156-11160 (1990).
Chou, "High-Resolution NMR Studies of Chimeric DNA-RNA-DNA Duplexes, Heteronomous Base Pairing, and Continuous Base Stacking at Junctions", Biochemistry 30:5248-5257 (1991).
Valencius and Smithies, "Double-Strand Gap Repair in a Mammalian Gene Targeting Reaction", Mol Cell Biol 11:4389 (1991).
Hendry et al., "A Ribozyme with DNA in the Hybridising Arms Displays Enhanced Cleavage Ability", Nuc Acids Res 20:5737-5741 (1992).
Taylor et al., "Chimeric DNA-RNA Hammerhead Ribozymes Have Enhanced in vitro Catalytic Efficiency and Increased Stability in vivo", Nuc Acids Res 20:4559-4565 (1992).
Usman et al., "Large Scale Chemical Synthesis, Purification and Cystallization of RNA-DNA Chimeras", Nuc Acids Res 20:6695-6699 (1992).
Usman and Cedergen, "Exploiting the Chemical Synthesis of RNA", Trends Bioch Sci 17:334-339 (1992).
Yamamoto et al., "Parameters Affecting the Frequencies of Transformation and Co-Transformation with Synthetic Oligonucleotides in Yeast", Yeast 8:935-948 (1992).
Egli et al., "Conformational Influence of the Ribose 2'-Hydroxyl Group: Crystal Structures of DNA-RNA Chimeric Duplexes", Biochemistry 32:3221-3227 (1993).
Sawata et al., "Enhancement of the Cleavage Rates of DNA-Armed Hammerhead Ribozymes by Various Divalent Metal Ions", Nuc Acids Res 21:5656-5660 (1993).
Shimayama, "Nuclease-Resistant Chimeric Ribozomes Containing Deoxyribonucleotides and Phosphorothioate Linkages", Nuc Acids Res 20:4559-4565 (1992).
Ban et al., "A Single 2'-Hydroxyl Group Converts B-DNA to A-DNA", J Mol Biol 236:275-285 (1994).
Salazar et al., "The Solution Structure of the r(gcg)d(TATACCC):d(GGGTATACGC) Ozaki Fragment Contains Two Distinct Duplex Morphologies Connected by a Junction", J Mol Biol 241:440-455 (1994).
Swiderski et al., "Polystyrene Reverse-Phase Ion-Pair Chromatography of Chimeric Ribozomes", Annal Biochem 216:83-88 (1994).
Brusca John S.
Friebel Thomas E.
Hansburg Daniel
Ketter James
Switzer Joan E.
LandOfFree
Compounds and methods for site directed mutations in eukaryotic does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Compounds and methods for site directed mutations in eukaryotic , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compounds and methods for site directed mutations in eukaryotic will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1959014