Compositions for detecting ras gene proteins and cancer therapeu

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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530395, 530413, 530416, 530828, C07K 102, C07K 118

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active

RE0351717

ABSTRACT:
Compositions useful for detecting ras gene proteins are described consisting of GTP and a protein having an apparent reduced molecular weight of about 115,000-120,000 daltons, or fragments derived therefrom, that stimulate ras protein guanosine triphosphatase activity. Also described are methods whereby the compositions are used to identify cancer therapeutics.

REFERENCES:
patent: 4404188 (1983-09-01), Donahoe et al.
Cales et al., Nature (England), 332 (6164) pp. 548-551, Apr. 7, 1988, "The Cytoplasmic Protein Gap is Implicated as the Target for Regulation by the Ras Gene Product".
Jackson B. Gibbs et al., 1988, PNAS (U.S.A.), 85:5026-5030.
McCormick et al., 1988, Cold Spring Harbor Symposia on Quantitative Biology, LIII:849-854, Cold Spring Harbor Laboratory.
Adari, H., et al., 1988, Science, 240:518-521.
Sigal, Irving S., 1988, Nature, 332:485-486.
McCormick, F. et al., 1988 CSH, (Abstract Only).
Trahey M. et al., (Abstract Only).

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